An Assessment of In-vivo and In-silico Diabetes Healing Potentialities of Terminalia chebula against Diverse Disturbed Pathological State in Experimental Rodent Model
Published: 2022-08-13
Page: 110-145
Issue: 2022 - Volume 5 [Issue 1]
Jannatul Ferdous
Department of Pharmaceutical Sciences, North South University, Plot # 15, Block # B, Bashundhara R/A, Dhaka 1229, Bangladesh.
Bushratul Jannat
Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.
Sanjida Jannath
Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.
Md. Mohin Uddin
Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.
Md. Rafat Tahsin
Department of Pharmaceutical Sciences, North South University, Plot # 15, Block # B, Bashundhara R/A, Dhaka 1229, Bangladesh.
Faisal Ahmed Nadvi
Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.
Nasiba Binte Bahar
Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Juhaer Anjum
Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Arifa Sultana
Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Ishrat Jahan
Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.
Fahima Aktar
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Jakir Ahmed Chowdhury
Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Abu Asad Chowdhury
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Md. Shah Amran
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Shaila Kabir *
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Terminalia chebula (T. chebula) is a widely used medicinal plant that possesses numerous therapeutic properties, such as antimicrobial, antioxidant, anti-diabetic, anti-inflammatory, hepatoprotective, cardioprotective activity. In this study, the ethanolic extract of T. chebula was observed to significantly improve the condition of alloxan-induced diabetic rats in a dose-dependent manner. A lower dose (250mg) of T. chebula significantly (p<0.05) reversed the altered physiological states of alloxan-induced diabetic rats, but a higher dose (650mg) yielded greater therapeutic effects. A dose-dependent restoration was also recorded in the levels of SGPT, SGOT, creatinine, HDL, LDL, and triglyceride levels in alloxan-induced diabetic rats that received three distinct doses (low, medium, high) of the test extract. Afterward, the diabetes healing potentialities of T. chebula were compared to those of commercially available medications. This study revealed that different doses of ethanolic extracts of T. chebula fruit had similar therapeutic results in treating hyperglycemia as existing conventional medications. A ligand library of the fruits’ constituents was prepared through literature mining, and the anti-diabetic activities of the ligands and their ADMET properties were assayed in silico. The molecular docking studies indicated that the anti-diabetic activity of the extract is likely mediated through the inhibition of α-amylase, α-glucosidase, and dipeptidyl peptidase-IV, but further research on this was deemed necessary. The current study ascertains the anti-diabetic potentialities of this medicinal plant and opines that comprehensive in vivo and in vitro analysis of the constituents be carried out to identify and further develop the actual molecules responsible for anti-diabetic activity.
Keywords: Diabetes mellitus, blood glucose, Terminalia chebula, lipid profile, alloxan, creatinine